A FACTORIAL DESIGN APPLIED TO THE STUDY OF CHROMIUM TOXICITY ON THE GLUTATHIONE LEVELS OF<i>Brachiaria brizantha</i>AND<i>Brachiaria ruziziensis</i>SEEDLINGS

W2183483752 · 수집 2026-10-04 18:12:22

T11342 Carbon and Quantum Dots Applications

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Chromium toxicity affects redox reactions within plant cells, generating detrimental reactive oxygen species.Glutathione is an antioxidant peptide and also a substrate for the production of phytochelatins, which are chelating peptides reported to mitigate Cr 3+ toxicity in plants.In this study, Brachiaria brizantha (B.brizantha) and Brachiaria ruziziensis (B.ruziziensis) seedlings were evaluated for physiological responses and glutathione production following the addition of zero or 5 mg L -1 Cr 3+ to the nutrient solution.Glutathione levels were determined by colorimetric analysis at 412 nm using 5,5'-dithio-bis(2-nitrobenzoic acid) as a chromophore reagent and recovery with glutathione reductase (with evaluations at days 10 and 20 of continuous growth).The assessments were carried out in a completely randomized design with 2 authentic replications, and arranged in a 2 3 factorial.Cr 3+ caused an average increase of 0.76 mg g -1 in the initial glutathione content.However, by day 20 there was an average reduction of 3.63 mg g -1 .Chromium-affected physiological detrimental responses, albeit detected in both species, were less-pronounced in B. ruziziensis, along with a much higher level of glutathione.This study indicates that B. ruziziensis has a greater tolerance for chromium toxicity than B. brizantha, and that glutathione is likely to be involved in the mitigation of chromium stress in B. ruziziensis.

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