The Determination of Dopamine in Presence of Serotonin on Dopamine-Functionalized Electrochemically Prepared Graphene Biosensor

W2132594289 · 수집 2026-10-04 18:12:21

T10660 Conducting polymers and applications

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Reported herein is the synthesis of electrochemically prepared graphene with dopamine (DA) functionalization and its application for the electrochemical detection of DA in presence of serotonin (5-HT). Graphene oxide-DA (GO-DA) resulted from the condensation reaction of GO and DA. It was characterized via several instrumental methods. The electrochemical reduction of GO-DA (ERGODA) has been employed for the electrochemical detection of DA without and with 5-HT. The electrochemical detection of DA has been verified through cyclic voltammetry, differential pulse voltammetry, and amperometric techniques in a 0.1 M phosphate buffer saline (PBS, pH 7.4). The influence of the accumulation time and pH of the PBS at ERGO-DA modified electrode for DA oxidation has been investigated. The interference has also been performed using interfering substances such as 5-HT, glucose, ascorbic acid, H2O2, and uric acid. The detection limit and linear range of DA have been calculated as 0.04 μM and 0.5‐100 μM, respectively, (where s/n = 3) at the ERGO-DA modified electrode. The real sample analysis has done without any difficulty and this biosensor can be used up to 10 days. © The Author(s) 2015. Published by ECS. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 License (CC BY, http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse of the work in any

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