<sup>13</sup> C magnetic resonance spectroscopy measurements with hyperpolarized [1‐ <sup>13</sup> C] pyruvate can be used to detect the expression of transgenic pyruvate decarboxylase activity in vivo

W1942878882 · 수집 2026-10-04 18:12:18

T11878 Solid-state spectroscopy and crystallography

CoT 라벨링

라벨링 전

PDF 파일

저장된 PDF 없음

기본 정보

DOI 제목 발행년도 저널 URL 페이지 열기 PDF URL PDF 열기 발행기관 피인용수 라이선스

추가 정보 초록

Purpose: Dissolution dynamic nuclear polarization can increase the sensitivity of the 13 C magnetic resonance spectroscopy experiment by at least four orders of magnitude and offers a novel approach to the development of MRI gene reporters based on enzymes that metabolize 13 C-labeled tracers.We describe here a gene reporter based on the enzyme pyruvate decarboxylase (EC 4.1.1.1),which catalyzes the decarboxylation of pyruvate to produce acetaldehyde and carbon dioxide.Methods: Pyruvate decarboxylase from Zymomonas mobilis (zmPDC) and a mutant that lacked enzyme activity were expressed using an inducible promoter in human embryonic kidney (HEK293T) cells.Enzyme activity was measured in the cells and in xenografts derived from the cells using 13 C MRS measurements of the conversion of hyperpolarized [1-13 C] pyruvate to H 13 CO - 3 .Results: Induction of zmPDC expression in the cells and in the xenografts derived from them resulted in an approximately two-fold increase in the H 13 CO - 3 /[1-13 C] pyruvate signal ratio following intravenous injection of hyperpolarized [1-13 C] pyruvate. Conclusion:We have demonstrated the feasibility of using zmPDC as an in vivo reporter gene for use with hyperpolarized 13 C MRS.

본문

PDF 본문 자동 추출 실패: [download] HTTP 403 Forbidden (2026-10-04 20:55:24)

활용가능성

이력 0건

아직 편집 이력이 없습니다. 저장할 때마다 이전 내용이 여기에 백업됩니다.