Selective nuclear localization of siRNA by metallic versus semiconducting single wall carbon nanotubes in keratinocytes

W1868374858 · 수집 2026-10-04 18:12:17

T10074 Carbon Nanotubes in Composites

CoT 라벨링

라벨링 전

PDF 파일

저장된 PDF 없음

기본 정보

DOI 제목 발행년도 저널 URL 페이지 열기 PDF URL 발행기관 피인용수 라이선스

추가 정보 초록

Background: The potential use of carbon nanotubes (CNTs) in gene therapy as delivery systems for nucleic acids has been recently recognized. Here, we describe that metallic versus semiconducting single-wall CNTs can produce significant differences in transfection rate and cellular distribution of siRNA in murine PAM212 keratinocytes. Results/Methodology: The results of cell interaction studies, coupled with supportive computational simulations and ultrastructural studies revealed that the use of metallic single wall CNTs resulted in siRNA delivery into both the cytoplasm and nucleus of keratinocytes, whereas semiconducting CNTs resulted in delivery only to the cytoplasm. Conclusion: Using enriched fractions of metallic or semiconducting CNTs for siRNA complex preparation may provide specific subcellular targeting advantages. Carbon nanotubes are a novel class of nanobuilding blocks extensively investigated as delivery systems for therapeutic RNAi to silence gene expression in the cell cytoplasm and nucleus. It was previously shown that the delivery properties of single wall carbon nanotubes (SWNTs) are influenced by their varied physicochemical characteristics, however, the effect of chirality and electronic properties of SWNTs have not been studied with respect to their role in cellular transfection. To the best of our knowledge, this is the first study that shows using metallic or semiconducting SWNTs for siRNA complex preparation may provide specific subcellular targeting advantages.

본문 활용가능성

이력 0건

아직 편집 이력이 없습니다. 저장할 때마다 이전 내용이 여기에 백업됩니다.